SK-BR-3-CAS9 cell line

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > Cas9 Stable Cell Lines > SK-BR-3-CAS9

EZ-editor™ Gene-editing Series Products

   CRISPR-iScreen™ Library

CRISPR-iScreen™ Library Plasmid

CRISPR-iScreen™ Library Virus

CRISPR-iScreen™ Cell Pool

   gRNA Plasmid Bank

   Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

   Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

   Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

   Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

SK-BR-3-CAS9 Order

SK-BR-3-CAS9 cell line
Catalog#
YC-C048-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
SK-BR-3-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
SK-BR-3-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the SK-BR-3 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a SK-BR-3-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
SK-BR-3-CAS9 Cas9 14.79796696 2.00070763
SK-BR-3-CAS9 β-actin 12.79725933
SK-BR-3 Cas9 33.42461395 20.89612579
SK-BR-3 β-actin 12.52848816
Cutting Efficiency Validation
SK-BR-3-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the SK-BR-3-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SK-BR-3		 STR Info (Cell bank)
Cell Line:SK-BR-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 912912
D13S317 11121112
D7S820 99912
D16S539 9999
VWA 17171717
TH01 8989
AMEL XXXX
TPOX 811811
CSF1PO 12121212
D12S391 1820
FGA 2020
D2S1338 2025
D21S11 3030.2
D18S51 1013
D8S1179 1212
D3S1358 1717
D6S1043 1119
PENTAE 1011
D19S433 1414
PENTAD 912
D1S1656 1117.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
SK-BR-3-CAS9 cell line
SK-BR-3-CAS9
Catalog#
YC-C048-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

EGFP Stable Cell Lines

Wild-type Cell Lines

Luciferase Stable Cell Lines

Related Popular Products

EGFP Stable Cell Lines

Wild-type Cell Lines

  • SK-BR-3 cell line

    Human Breast Adenocarcinoma Cell Line(SK-BR-3)

    Price (USD): -
    Catalog#: YC-C048
    Size: 1*10^6
    Instruction: Human Breast Adenocarcinoma Cell Line(SK-BR-3)
      inquiry   More details

Luciferase Stable Cell Lines


WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us